Radio-adaptive response of peripheral blood lymphocytes following bystander effects induced by preirradiated CHO-K1 cells using the micronucleus assay

radio-adaptive response of peripheral blood lymphocytes following bystander effects induced by preirradiated cho-k1 cells using the micronucleus assay

background: radio-adaptive response and bystander effects are known phenomena occurring in cells following exposure to ionizing radiation (ir). in this study we examined possible radio-adaptation of lymphocytes following bystander effects induced by cho-k1 cells. materials and methods: whole blood and cho-k1 cells were cultured in rpmi-1640 complete medium. cells were separately irradiated with...

Dose estimation with the calibration of dose-response curve of micronucleus in human peripheral lymphocytes induced by 50MeV proton beams

Background: The purpose of this paper is to establish an easy and reliable biodosimeter protocol to evaluate the biological effects of proton beams. Materials and Methods: Human peripheral blood lymphocytes were irradiated using proton beams (LET: 34.6 keV μm−1), and the chromosome aberrations induced were analyzed using cytokinesis-blocked (CB) micronucleus (MN) assay. To determine th...

Evaluation of an automated in vitro micronucleus assay in CHO-K1 cells.

In this paper, we describe the evaluation of an automated in vitro micronucleus assay using CHO-K1 cells in 96-well plates. CHO-K1 cells were pre-loaded with a cell dye that stains the cytoplasm, after which the cells were treated with the test compounds for either 3h (for the +S9 condition) or 24h (for the -S9 condition). A total of 10 concentrations were tested, of which the top five concentr...

The Role of Target and Bystander Cells in Dose-Response Relationship of Radiation-Induced Bystander Effects in Two Cell Lines

Cytogenetic analysis of Greek farmers using the micronucleus assay in peripheral lymphocytes and buccal cells.

The potential cytogenetic damage associated with pesticide use in Greek agricultural workers was evaluated using micronuclei (MN) as biomarkers in lymphocytes of peripheral blood and exfoliated cells of the buccal mucosa. In addition, the effects of pesticide exposure and other variables on the cytokinesis block proliferation index (CBPI) in lymphocytes were also evaluated. Both the exposed and...

Radioprotective effects of selenium and vitamin-E against 6MV X-rays in human blood lymphocytes by micronucleus assay

Background: Critical macromolecules of cells such as DNA are in exposure to damage of free radicals that induced from the interaction of ionizing radiation with biological systems. Selenium and vitamin-E are natural compounds that have been shown to be a direct free radical scavenger. The aim of this study was to investigate the radioprotective effect of selenium and vitamin-E separately and sy...